The objective for this project was to determine what compounds were uniquely generated from skin treated with lotion. Samples (conditioned Twister bars exposed to skin headspace) were received from Unilever and stored under refrigeration until analyzed by a GC-MSD equipped with Gerstel components including: MPS2, TDU, CIS, and MACH. Twister bars were placed into TDU tubes and heated to 300oC for 4 minutes inside a thermal desorption unit (TDU). The TDU transfer temp was 300oC and operated in splitless mode with purge time 1.00. Volatiles were swept onto a cryo-cooled (LN2) inlet liner at -130oC (CIS4 liner in split mode 30:1). The liner contained glass beads for increased adsorption. The liner was heated to 300oC for 3 min holding time and volatiles were transferred to the analytical DB-5 MACH column. Column heating conditions, two ramp rates, were: held at 35oC for 5 min then 4oC/min to 220oC for 0 min holding time and 7°C/min to 300°C for 1 min holding time. Total ion chromatogram time was 63.68 min. Conditions were ramped pressure with initial pressure 46.9 kPa for 5 min then 1.6 kPa/min to 120.0 kPa for 0 min and 2.8 kPa/min to 151.4 kPa for 1 min. MSD transfer line temperature 280oC. Scan parameters were 35-350 a.m.u. The DB-5 capillary column utilized was a 30m x 0.25mm I.D. x 0.25µm film thickness. Components were identified by matching spectra with a Wiley 2007 library.